Quantitation of the main constituents of vanilla by reverse phase HPLC and ultra-high-pressure-liquid-chromatography with UV detection: method validation and performance comparison.

@article{Cicchetti2009QuantitationOT,
  title={Quantitation of the main constituents of vanilla by reverse phase HPLC and ultra-high-pressure-liquid-chromatography with UV detection: method validation and performance comparison.},
  author={Esmeralda Cicchetti and Alain Chaintreau},
  journal={Journal of separation science},
  year={2009},
  volume={32 17},
  pages={3043-52}
}
Vanilla's main constituents, i. e., vanillin, para-hydroxybenzaldehyde, and their corresponding acids, can be easily quantified by RP LC with UV detection and external calibration. This paper describes two methods that were developed using HPLC and ultra-high-pressure LC (UHPLC), respectively, and validated according to the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH). Both methods were highly specific, exhibited… CONTINUE READING

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