Quantitation of secretory group V phospholipase A(2) in human tissues by sandwich enzyme-linked immunosorbent assay.

@article{Muoz2002QuantitationOS,
  title={Quantitation of secretory group V phospholipase A(2) in human tissues by sandwich enzyme-linked immunosorbent assay.},
  author={Nilda M. Mu{\~n}oz and Evan E. Boetticher and Anne I Sperling and Kwang-Pyo Kim and Angelo Y. Meliton and Xiangdong Zhu and Anissa Therese Lambertino and Wonhwa Cho and Alan R. Leff},
  journal={Journal of immunological methods},
  year={2002},
  volume={262 1-2},
  pages={
          41-51
        }
}
We have developed a sensitive sandwich ELISA (sELISA) for quantitative determination of group V phospholipase A(2) (gVPLA(2)). This assay utilizes three monoclonal antibodies (mAbs) directed against human gVPLA(2) (MCL-1B7, MCL-2A5, and MCL-3G1), which recognize specifically different epitopes of gVPLA(2). A mixture of MCL-1B7 and MCL-2A5 was used as the capture mAb, and MCL-3G1 as the detector mAb; purified human gVPLA(2) was used as the standard protein. The limit of detection of the sELISA… CONTINUE READING
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