Quantitation of ranaviruses in cell culture and tissue samples.

  title={Quantitation of ranaviruses in cell culture and tissue samples.},
  author={Riikka L Holopainen and Jarno Honkanen and Britt Bang Jensen and Ellen Ariel and Hannele Tapiovaara},
  journal={Journal of virological methods},
  volume={171 1},
A quantitative real-time PCR (qPCR) based on a standard curve was developed for detection and quantitation of ranaviruses. The target gene for the qPCR was viral DNA polymerase (DNApol). All ten ranavirus isolates studied (Epizootic haematopoietic necrosis virus, EHNV; European catfish virus, ECV; European sheatfish virus, ESV; Frog virus 3, FV3; Bohle… CONTINUE READING