Quantitation of pyrimethamine and related diaminopyrimidines in situ by enhancement of fluorescence after thin layer chromatography.

  title={Quantitation of pyrimethamine and related diaminopyrimidines in situ by enhancement of fluorescence after thin layer chromatography.},
  author={W. S. Simmons and R. Deangelis},
  journal={Analytical chemistry},
  volume={45 8},
13 Citations
Luminescence Methods in Drug Analysis
Investigation of silver iodide-based ion-selective membranes by scanning electrochemical microscopy
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A rapid, sensitive and selective method was developed for the simultaneous determination of pyrimethamine and mefloquine, two of the active ingredients of Fansimef, in human plasma and the practicability of the method was demonstrated by the analysis of more than 1200 plasma samples from several pharmacokinetic studies involving single-dose administration of fansimef to both patients and volunteers. Expand
Analytical Methods in Human Toxicology
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The mechanism of pyrimethamine resistance in Plasmodium falciparum.
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Simple and sensitive spectrofluorimetric determination of pyrimethamine in biological fluids
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Dihydrofolate Reductase Inhibitors
While exploring structural variations of anilinopyrimidines with antimalarial activity, a group at Imperial Chemical Industries found that certain biguanides synthesised as ring-opened analoguesExpand
Surface studies on precipitate-based cyanide electrodes
Abstract Silver iodide-based electrodes allow indirect measurements of cyanide. Potentiometric investigations and theoretical studies have suggested that a corrosion process is responsible for thisExpand
Determination of pyrimethamine in human plasma after administration of fansidar of fansidar-mefloquine by means of high-performance liquid chromatography with fluorescence detection.
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Quantitative high-pressure liquid chromatographic procedure for the determination of plasma and tissue levels of 2,4-diamino-5-(3,4-dichlorophenyl)-6-methylpyrimidine (metoprine) and its application to the measurement of brain capillary permeability coefficients.
A high-pressure liquid chromatographic assay for metoprine levels in aqueous media, serum, and tissue extracts has been developed that can measure concentrations of the drug in the 25-100 ng rangeExpand


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Treatment of meningeal leukemia with pyrimethamine.
Considerations regarding lipid solubility, body distribution, and easily reversible toxicity of pyrimethamine suggest that further therapeutic trials are warranted, especially in childhood lymphoblastic leukemia. Expand
Detection and fluorescent measurement of pyrimethamine in urine
Abstract A method is described for the measurement of pyrimethamine in urine using the natural fluorescence of the compound. An extract from the urine is chromatographed on a flexible thin-layerExpand
The pharmacological properties of 2, 4-diamino-5-p-chlorophenyl-6-ethylpyrimidine, daraprim.
This investigation has dealt with the absorption, excretion, and metabolism of Daraprim in the rhesus monkey and with the tissue distribution and toxicity of this drug in both the monkey and theExpand