Quantitation of prenylcysteines by a selective cleavage reaction.

  title={Quantitation of prenylcysteines by a selective cleavage reaction.},
  author={William W. Epstein and D C Lever and L M Leining and Eveline Bruenger and H C Rilling},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={88 21},
The allylic thioether bond of the prenylcysteines of prenylated proteins has been shown to be cleaved by 2-naphthol under alkaline conditions to yield substituted naphthopyrans. These products are readily resolved from interfering materials by HPLC and have a strongly absorbing chromophore. Thus, this reaction is suitable for quantitative analysis of prenyl substituents of proteins, and we have examined a number of tissues for their content of prenylcysteines. These amino acids are present in… CONTINUE READING
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