Quantitation of plasma membrane calcium pump phosphorylated intermediates by electrophoresis.

Abstract

P-ATPases are characterized by the formation of acid-stable phosphorylated intermediates (EP) during their reaction cycle. We have developed a microscale method to determine EP that involves the phosphorylation of the enzyme using [gamma-(32)P]ATP and precipitation with TCA; separation of the sample by SDS-PAGE, and measurement of the enzyme protein and (32… (More)

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Cite this paper

@article{Echarte2001QuantitationOP, title={Quantitation of plasma membrane calcium pump phosphorylated intermediates by electrophoresis.}, author={Mar{\'i}a Mercedes Echarte and Valeria Levi and Alejandro M. Villamil and R. Rossi and J. P. Rossi}, journal={Analytical biochemistry}, year={2001}, volume={289 2}, pages={267-73} }