Quantitation of indoleacetic Acid conjugates in bean seeds by direct tissue hydrolysis.

@article{Bialek1989QuantitationOI,
  title={Quantitation of indoleacetic Acid conjugates in bean seeds by direct tissue hydrolysis.},
  author={K Bialek and Jerry D. Cohen},
  journal={Plant physiology},
  year={1989},
  volume={90 2},
  pages={
          398-400
        }
}
Gas chromatography-selected ion monitoring-mass spectral analysis using [(13)C(6)]indole-3-acetic acid (IAA) as an internal standard provides an effective means for quantitation of IAA liberated during direct strong basic hydrolysis of bean (Phaseolus vulgaris L.) seed powder, provided that extra precautions are undertaken to exclude oxygen from the reaction vial. Direct seed powder hydrolysis revealed that the major portion of amide IAA conjugates in bean seeds are not extractable by aqueous… CONTINUE READING
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