Quantitation of human protein S in the plasma of normal and warfarin-treated individuals by radioimmunoassay.

@article{Fair1984QuantitationOH,
  title={Quantitation of human protein S in the plasma of normal and warfarin-treated individuals by radioimmunoassay.},
  author={Daryl S. Fair and D J Revak},
  journal={Thrombosis research},
  year={1984},
  volume={36 6},
  pages={
          527-35
        }
}
Protein S was purified from human plasma and used to raise monospecific antibodies. A double antibody equilibrium radioimmunoassay was constructed and met all the requirements for a sensitive, accurate and specific determination of Protein S. The sensitivity of the assay was between 8-250 ng of Protein S/ml and the coefficient of variation was 2-6% within assays and 13-19% between assays. Complement C4b-binding protein or Protein C did not effect the measurement of Protein S. Complete… CONTINUE READING
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