Quantitation of hepatitis B virus genomic DNA by real-time detection PCR.

@article{Abe1999QuantitationOH,
  title={Quantitation of hepatitis B virus genomic DNA by real-time detection PCR.},
  author={Aki Abe and Kazuaki Inoue and Tatsuya Tanaka and Junko Isono Kato and Naoki Kajiyama and Ryuji Kawaguchi and S Tanaka and M Yoshiba and Michinori Kohara},
  journal={Journal of clinical microbiology},
  year={1999},
  volume={37 9},
  pages={
          2899-903
        }
}
Quantitation of hepatitis B virus (HBV) DNA in serum is a useful method for the monitoring of HBV replication. We attempted to develop a quantitative assay system for HBV DNA that is more sensitive, accurate, and reproducible than existing systems. We detected HBV DNA by real-time detection PCR (RTD-PCR) based on Taq Man chemistry. The efficacy of this assay was evaluated by quantitatively measuring sequential levels of synthetic DNA and DNA in clinical serum samples. The detection limit of… CONTINUE READING

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