Quantitation of genomic DNA in plasma and serum samples: higher concentrations of genomic DNA found in serum than in plasma

@article{Lee2001QuantitationOG,
  title={Quantitation of genomic DNA in plasma and serum samples: higher concentrations of genomic DNA found in serum than in plasma},
  author={Tzong-Hae Lee and Leilani Montalvo and Vera Chrebtow and Michael P. Busch},
  journal={Transfusion},
  year={2001},
  volume={41}
}
BACKGROUND: Plasma and serum samples have been used to detect cell‐free genomic DNA in serum or plasma in certain pathologic conditions such as systemic lupus erythematosus, pulmonary embolism, and malignancies, as well as in fetal cell chimerisms in maternal serum and/or plasma. In this study, baseline concentrations of cell‐free DNA in serum and plasma samples were evaluated for the study of posttransfusion chimerism. 
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302 Citations
Highly Influential Citations
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Background Citations
108
Methods Citations
12
Results Citations
4

302 Citations

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Citation Type

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References

SHOWING 1-10 OF 26 REFERENCES
Presence of fetal DNA in maternal plasma and serum
Free DNA in serum and plasma from normal adults.
  • C. Steinman
  • Biology, Medicine
    The Journal of clinical investigation
  • 1975
TLDR
It is concluded that the appearance of DNA in adult human plasma is a pathological event and it is emphasized that the use of serum in studies dependent on sensitive assays for DNA (or anti-DNA antibody) introduces an ambiguity that may be avoided by substitution of carefully collected plasma for serum.
Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis.
We have developed a real-time quantitative PCR assay to measure the concentration of fetal DNA in maternal plasma and serum. Our results show that fetal DNA is present in high concentrations in…
The elevation of plasma DNA in patients with systemic lupus erythematosus is attributable to increased DNA release and defective DNA binding of mononuclear cells.
TLDR
The results suggest that some subsets of lymphocytes can be sensitized by different DNA samples in vivo to increase endogenous DNA release from mononuclear cells, which in addition to decreased DNA clearance as has been previously reported, may be responsible for the elevation of plasma DNA in patients with SLE.
Cell-free DNA in human blood plasma: length measurements in patients with pancreatic cancer and healthy controls.
TLDR
Evidence is provided indicating differences in non-cell-associated DNA in plasma between cancer patients and healthy controls and indicates that a significant amount of this DNA is probably derived from apoptosis in neoplastic and/or normal cells.
[Plasma DNA level as a tumor marker in primary lung cancer].
  • A. Maebo
  • Medicine, Biology
    Nihon Kyobu Shikkan Gakkai zasshi
  • 1990
TLDR
In the patients with lung cancer who responded to treatment, the plasma DNA levels were significantly decreased after treatment, while its levels were elevated in the patients whose treatment was unsuccessful, indicating that plasma DNA may be a useful marker in patients with Lung cancer.
Rearranged Ig heavy chain DNA is detectable in cell-free blood samples of patients with B-cell neoplasia.
TLDR
It is suggested that tumor-derived DNA can be detected in serum or plasma of the majority of patients with B-cell malignancies and that testing of serum or Plasma for tumor-associated DNA may be a novel parameter for monitoring response to treatment.
Detection of circulating DNA in plasma of patients with pulmonary embolism by counterimmunoelectrophoresis.
TLDR
Counterimmunoelectrophoresis is a rapid, inexpensive and specific test which may be used as an exclusion test in the detection of patients suspected of having PE, who will have to undergo more extensive evaluation.
Isolation and characterization of DNA from the plasma of cancer patients.
Neoplastic characteristics of the DNA found in the plasma of cancer patients.
About one third of patients with various malignant diseases were found to have extractable amounts of DNA in their plasma whereas no DNA could be detected in normal controls. Using the test…
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