Quantitation of genomic DNA in plasma and serum samples: higher concentrations of genomic DNA found in serum than in plasma

  title={Quantitation of genomic DNA in plasma and serum samples: higher concentrations of genomic DNA found in serum than in plasma},
  author={Tzong-Hae Lee and Leilani Montalvo and Vera Chrebtow and Michael P. Busch},
BACKGROUND: Plasma and serum samples have been used to detect cell‐free genomic DNA in serum or plasma in certain pathologic conditions such as systemic lupus erythematosus, pulmonary embolism, and malignancies, as well as in fetal cell chimerisms in maternal serum and/or plasma. In this study, baseline concentrations of cell‐free DNA in serum and plasma samples were evaluated for the study of posttransfusion chimerism. 
Changes in concentration of DNA in serum and plasma during storage of blood samples.
The influence of time delay in blood processing for plasma and serum at room temperature and at 4 °C is analyzed to complement the data of Lui et al. (5).
Quantification of Circulating DNA in the Plasma and Serum of Cancer Patients
Findings provide conclusive evidence that elevated levels of circulating DNA could be identified consistently in patients with cancer than in normal healthy donors, and the method of blood processing may significantly affect the Levels of circulating nucleic acids and impact the investigator's results.
Is the quantity of circulatory cell-free DNA in human plasma and serum samples associated with gender, age and frequency of blood donations?
The relationship between the level of circulatory cf-DNA and physiological parameters such as gender, age and frequency of blood donations, and no significant differences were observed between the age-matched men and women and gender-matched, different-age cohorts, except in women who were older than 60 years of age.
Analysis of methylated circulating DNA in cancer patients' blood.
Circulating extracellular nucleic acids derived from body fluids such as blood are commonly analyzed to assess malignant diseases. Efficient isolation, extraction, quantification, modification, and
Isolation and Extraction of Circulating Tumor DNA from Patients with Small Cell Lung Cancer
It is concluded that plasma is a more reliable source of tumor DNA then serum and a robust procedure for plasma tumor DNA isolation that can be applied to translational research studies.
Extracellular DNA in cord blood plasma and applications in cord blood banking for sample identification
It is hypothesized that human genomic DNA may also be present in CB plasma, similar to viral DNA sequences present in the CB plasma of carrier babies.
Effects of prolonged storage of whole plasma or isolated plasma DNA on the results of circulating DNA quantification assays.
Analysis of molecular markers in biological fluids has been proposed as a tool for early detection and monitoring of cancer and provides valuable information for the rational planning of retrospective studies of banked series of biological samples, particularly if collected over a long period of time, as can occur in large clinical trials.
Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction
It is suggested that using plasma minimizes the dilution of tumor-derived DNA and optimizes the sensitivity of ct DNA analysis, potentially contributing to ctDNA analysis standardization.


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  • Biology, Medicine
    The Journal of clinical investigation
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It is concluded that the appearance of DNA in adult human plasma is a pathological event and it is emphasized that the use of serum in studies dependent on sensitive assays for DNA (or anti-DNA antibody) introduces an ambiguity that may be avoided by substitution of carefully collected plasma for serum.
Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis.
We have developed a real-time quantitative PCR assay to measure the concentration of fetal DNA in maternal plasma and serum. Our results show that fetal DNA is present in high concentrations in
The elevation of plasma DNA in patients with systemic lupus erythematosus is attributable to increased DNA release and defective DNA binding of mononuclear cells.
The results suggest that some subsets of lymphocytes can be sensitized by different DNA samples in vivo to increase endogenous DNA release from mononuclear cells, which in addition to decreased DNA clearance as has been previously reported, may be responsible for the elevation of plasma DNA in patients with SLE.
Cell-free DNA in human blood plasma: length measurements in patients with pancreatic cancer and healthy controls.
Evidence is provided indicating differences in non-cell-associated DNA in plasma between cancer patients and healthy controls and indicates that a significant amount of this DNA is probably derived from apoptosis in neoplastic and/or normal cells.
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  • A. Maebo
  • Medicine, Biology
    Nihon Kyobu Shikkan Gakkai zasshi
  • 1990
In the patients with lung cancer who responded to treatment, the plasma DNA levels were significantly decreased after treatment, while its levels were elevated in the patients whose treatment was unsuccessful, indicating that plasma DNA may be a useful marker in patients with Lung cancer.
Rearranged Ig heavy chain DNA is detectable in cell-free blood samples of patients with B-cell neoplasia.
It is suggested that tumor-derived DNA can be detected in serum or plasma of the majority of patients with B-cell malignancies and that testing of serum or Plasma for tumor-associated DNA may be a novel parameter for monitoring response to treatment.
Detection of circulating DNA in plasma of patients with pulmonary embolism by counterimmunoelectrophoresis.
Counterimmunoelectrophoresis is a rapid, inexpensive and specific test which may be used as an exclusion test in the detection of patients suspected of having PE, who will have to undergo more extensive evaluation.
Isolation and characterization of DNA from the plasma of cancer patients.
Neoplastic characteristics of the DNA found in the plasma of cancer patients.
About one third of patients with various malignant diseases were found to have extractable amounts of DNA in their plasma whereas no DNA could be detected in normal controls. Using the test