Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.

@article{Wu1981QuantitationOC,
  title={Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.},
  author={Wen-Jin Wu and Harvey A. Moore and Michael A. Raftery},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1981},
  volume={78 2},
  pages={775-9}
}
A stopped-flow spectroscopic technique was used to study the kinetics of ion transport by reconstituted membrane preparations containing purified acetylcholine receptor. Influx of thallium (I) into membrane vesicles was monitored as a decrease, due to quenching by the thallous ion, in the fluorescence of an entrapped fluorophore. In a reproducible manner, the reconstituted receptor responded to cholinergic agonists by mediating rapid ion transport in the millisecond time range. The effect of… CONTINUE READING