Quantitation of biogenic tetraamines in Arabidopsis thaliana.

  title={Quantitation of biogenic tetraamines in Arabidopsis thaliana.},
  author={Jose Luis Rambla and Francisco Vera-Sirera and Miguel Angel Bl{\'a}zquez and Juan Manuel Carbonell and Antonio Granell},
  journal={Analytical biochemistry},
  volume={397 2},
Classical methods for the determination of polyamines in Arabidopsis, based on high-performance liquid chromatography or thin-layer chromatography, do not distinguish between aliphatic tetraamines or do not allow accurate quantitation, respectively. Here we describe a gas chromatography-mass spectrometry method that allows separation and simple quantitation of spermine and thermospermine. The reliability of the protocol has been confirmed using extracts of Arabidopsis mutants impaired in… CONTINUE READING