Quantitation of Taura syndrome virus by real-time RT-PCR with a TaqMan assay.

@article{Tang2004QuantitationOT,
  title={Quantitation of Taura syndrome virus by real-time RT-PCR with a TaqMan assay.},
  author={Kathy F.-J. Tang and Jun Wang and Donald V. Lightner},
  journal={Journal of virological methods},
  year={2004},
  volume={115 1},
  pages={109-14}
}
A real-time RT-PCR assay was developed using a TaqMan probe to detect and quantify Taura syndrome virus (TSV) in penaeid shrimp. A pair of RT-PCR primers, which amplify a 72 bp DNA fragment, and a TaqMan probe were selected from open reading frame 1 (ORF1) of the TSV genome. The primers and TaqMan probe used in this assay reacted with TSV isolates from Hawaii, Texas, Colombia, Mexico, Belize, Indonesia, and Thailand, but neither with RNA of healthy shrimp nor with an isolate of yellow head… CONTINUE READING

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