Quantitation of TGF-beta1 mRNA in porcine mesangial cells by comparative kinetic RT/PCR: comparison with ribonuclease protection assay and in situ hybridization.

@article{Ceol2001QuantitationOT,
  title={Quantitation of TGF-beta1 mRNA in porcine mesangial cells by comparative kinetic RT/PCR: comparison with ribonuclease protection assay and in situ hybridization.},
  author={Monica Ceol and Monica Forino and Giovanni Gambaro and Ulrich Sauer and Erwin D. Schleicher and Angela D'Angelo and Franca Anglani},
  journal={Journal of clinical laboratory analysis},
  year={2001},
  volume={15 4},
  pages={215-22}
}
Gene expression can be examined with different techniques including ribonuclease protection assay (RPA), in situ hybridisation (ISH), and quantitative reverse transcription-polymerase chain reaction (RT/PCR). These methods differ considerably in their sensitivity and precision in detecting and quantifying low abundance mRNA. Although there is evidence that RT/PCR can be performed in a quantitative manner, the quantitative capacity of this method is generally underestimated. To demonstrate that… CONTINUE READING
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