Quantitation of RNA polymerase subunits in Escherichia coli during exponential growth and after bacteriophage T4 infection


A method was developed to measure the amounts of RNA polymerase subunits, α, β, β′ and σ in crude extracts of Escherichia coli. The proteins were labelled by growing the cells in 35S-sulphate containing media. For measuring β and β′, the cell lysate was electrophoresed on 6% polyacrylamide gels containing SDS and the β and β′ bands cut out and counted. For… (More)
DOI: 10.1007/BF00269405

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