Quantitation of DNA methylation in Epstein-Barr virus–associated nasopharyngeal carcinoma by bisulfite amplicon sequencing

  title={Quantitation of DNA methylation in Epstein-Barr virus–associated nasopharyngeal carcinoma by bisulfite amplicon sequencing},
  author={Weilin Zhao and Yingxi Mo and Shumin Wang and Kaoru Midorikawa and Ning Ma and Yusuke Hiraku and Shinji Oikawa and Guangwu Huang and Zhe Zhang and Mariko Murata and Kazuhiko Takeuchi},
  journal={BMC Cancer},
BackgroundEpigenetic changes, including DNA methylation, disrupt normal cell function, thus contributing to multiple steps of carcinogenesis. Nasopharyngeal carcinoma (NPC) is endemic in southern China and is highly associated with Epstein-Barr virus (EBV) infection. Significant changes of the host cell methylome are observed in EBV-associated NPC with cancer development. Epigenetic marks for NPC diagnosis are urgently needed. In order to explore DNA methylation marks, we investigated DNA… 
Combination of RERG and ZNF671 methylation rates in circulating cell‐free DNA: A novel biomarker for screening of nasopharyngeal carcinoma
The methylation rates of specific genes in ccfDNA can serve as novel biomarkers for early detection and screening of NPC.
Modulation of Cellular CpG DNA Methylation by Kaposi's Sarcoma-Associated Herpesvirus
It is shown that treating PEL cells with a demethylating drug (5-aza-2′-deoxycytidine) led to inhibition of cell growth, raising the possibility of testing this drug for the treatment of PEL, and that DNA methylation plays a role in the repression of these human genes.
DNA methylation biomarkers for nasopharyngeal carcinoma
Novel aberrantly methylated-differentially expressed genes and pathways in NPC are revealed by bioinformatics analysis, potentially providing novel insights for the molecular mechanisms governing NPC progression.
Epstein-Barr Virus-Induced Epigenetic Pathogenesis of Viral-Associated Lymphoepithelioma-Like Carcinomas and Natural Killer/T-Cell Lymphomas
Recent advances of epigenetic alterations in Epstein-Barr virus-associated tumors (LELCs and NKTCL) are summarized, highlighting the importance of epigenetics etiology in EBV-associated tumorigenesis.
Predictive Value of CD8 Expression and FoxP3 Methylation in Nasopharyngeal Carcinoma Patients Treated with Chemoradiotherapy in a Non-endemic Area
The data suggest that a high level of immunosuppression in the UNPC microenvironment may predict treatment failure and may allow an early identification of patients who could benefit from the addition of immune modulating strategies to improve first line CRT.
The Impact of Epstein-Barr Virus Infection on Epigenetic Regulation of Host Cell Gene Expression in Epithelial and Lymphocytic Malignancies
This review summarizes the currently available epigenetic profiles of several well-studied EBV-associated cancers and the relevant distinct mechanisms leading to aberrant epigenetic signatures due to EBV.
Hypermethylation of SHISA3 Promotes Nasopharyngeal Carcinoma Metastasis by Reducing SGSM1 Stability.
It is reported here that promoter hypermethylation of SHISA3 is common and contributes to the downregulation of this gene in many types of tumors, including NPC, and this newly identified SHISA-TRIM21-SGSM1 axis could be a novel therapeutic target in NPC.
Epigenetic-Mediated Downregulation of Zinc Finger Protein 671 (ZNF671) Predicts Poor Prognosis in Multiple Solid Tumors
Pan-cancer analysis indicates that ZNF671 is an excellent predictive factor for BRCA, CESC, HNSC, KIRP, LUAD, PAAD, SARC, and UCEC solid tumors and may play crucial roles in the development and progression of these tumors.
Transcriptomic Analyses Reveal Gene Expression Profiles and Networks in Nasopharyngeal Carcinoma
Information is provided on the genes that may be involved in the development of NPC and suggests possible druggable targets and biomarkers for diagnosing and evaluating the prognosis of NPC.
Metabolic Reprogramming and Immune Evasion in Nasopharyngeal Carcinoma
This review presents the dysregulated metabolism processes, including glucose, fatty acid, amino acid, nucleotide metabolism, and their mutual interactions in NPC and the potential connections between reprogrammed metabolism, tumor immunity, and associated therapy.


Methylomics analysis identifies ZNF671 as an epigenetically repressed novel tumor suppressor and a potential non-invasive biomarker for the detection of urothelial carcinoma
ZNF671, an epigenetically silenced novel tumor suppressor, represents a potential predictor for UC relapse and non-invasive biomarker that could assist in UC clinical decision-making.
Genetic and epigenetic landscape of nasopharyngeal carcinoma.
An updated overview of the genetic and epigenetic aberrations that likely drive nasopharyngeal tumor development and progression is provided and the potential use of these findings to identify biomarkers for NPC diagnosis and prognosis is discussed.
Downregulation of ATOH8 induced by EBV-encoded LMP1 contributes to the malignant phenotype of nasopharyngeal carcinoma
This study uncovered the occurrence of malignant phenotype of NPC induced by EBV infection and characterized a novel bHLH transcription factor ATOH8 as a new downstream target of LMP1.
DNA Hypermethylation of SHISA3 in Colorectal Cancer: An Independent Predictor of Poor Prognosis
The quantitative bisulfite pyrosequencing assay established could be a cost-effective tool for providing a potential biomarker of adverse prognosis in CRC and is of prognostic significance in predicting clinical outcome.
Genome-scale DNA methylation maps of pluripotent and differentiated cells
Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.
Molecular and cytogenetic changes involved in the immortalization of nasopharyngeal epithelial cells by telomerase
The deletion of the p16INK4A locus appears to be the most crucial event for the immortalization of nasopharyngeal epithelial cells by telomerase and precedes RASSF1A downregulation, which may play a role in the survival of immortalized NP epithel cells.
Inactivation of RASSF2A by promoter methylation correlates with lymph node metastasis in nasopharyngeal carcinoma
RASSF2A, a candidate tumor suppressor gene (TSG), is frequently inactivated by its promoter methylation and this aberrant methylation correlates with lymph node metastasis in NPC.
Nasopharyngeal carcinoma: molecular pathogenesis and therapeutic developments
  • Q. Tao, A. Chan
  • Biology, Medicine
    Expert Reviews in Molecular Medicine
  • 2007
New biomarkers for NPC, including EBV DNA copy number or methylation of multiple tumour suppressor genes, which can be detected in serum and nasopharyngeal brushings, have been developed for the molecular diagnosis of this tumour.
Promoter hypermethylation of Ras-related GTPase gene RRAD inactivates a tumor suppressor function in nasopharyngeal carcinoma.