Quantitation of DNA

  title={Quantitation of DNA},
  author={Philippe Sauer and Markus M{\"u}ller and Jie Kang},
Materials and methods All spectrophotometric measurements were taken in a Beckman DU7400 or DU640 spectrophotometer using a reference containing the appropriate solvent but without DNA. The solvent used was 10 mM Tris·Cl, pH 8.5 unless otherwise stated. Measurements were repeated 4 times. Experiments concerning the reliability of A260 values and the effects of solvents were performed using plasmid DNA purified with the QIAGEN® Plasmid Maxi Kit. Experiments investigating the effects of RNA… CONTINUE READING