Quantitation of DEFA1A3 gene copy number polymorphism by allele specific amplification and real-time PCR

@inproceedings{Nmeth2013QuantitationOD,
  title={Quantitation of DEFA1A3 gene copy number polymorphism by allele specific amplification and real-time PCR},
  author={Bal{\'a}zs N{\'e}meth and {\'A}d{\'a}m Horv{\'a}th and M{\'a}rta Szekeres and Yvette M{\'a}ndi and Ferenc Somogyv{\'a}ri},
  year={2013}
}
Some of the PCR based genotyping methods are faster and less expensive than sequencing in population-wide studies. One of the cost effective solutions is the allele specific amplification (ASA). We applied this method for quantitative analysis of defensin A1 (DEFA1) and defensin A3 (DEFA3) genes which are known to have copy number polymorphism in the human genome. The proteins encoded by these genes are human alpha defensins / human neutrophil peptides 1 and 3. Their antimicrobial mechanisms… CONTINUE READING

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