Quantitation of 25-OH-vitamin D (25OHD) using liquid tandem mass spectrometry (LC-MS-MS).

25-OH-vitamin D (25OHD) is ordered and interpreted clinically in light of calcium balance and homeostasis. Various methodologies including immunoassay and chromatography are described for the measurement of 25OHD in biological fluids. It is well reported that all existing methods are challenging and require a high level of technical expertise. While this is… CONTINUE READING