Quantifying the dynamic interactions between a clathrin-coated pit and cargo molecules.

@article{Weigel2013QuantifyingTD,
  title={Quantifying the dynamic interactions between a clathrin-coated pit and cargo molecules.},
  author={Aubrey V. Weigel and Michael M. Tamkun and Diego Krapf},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2013},
  volume={110 48},
  pages={E4591-600}
}
Clathrin-mediated endocytosis takes place through the recruitment of cargo molecules into a growing clathrin-coated pit (CCP). Despite the importance of this process to all mammalian cells, little is yet known about the interaction dynamics between cargo and CCPs. These interactions are difficult to study because CCPs display a large degree of lifetime heterogeneity and the interactions with cargo molecules are time dependent. We use single-molecule total internal reflection fluorescence… CONTINUE READING