Quantifying the Interaction between Copper-Zinc Superoxide Dismutase (Sod1) and its Copper Chaperone (Ccs1)

@inproceedings{Boyd2018QuantifyingTI,
  title={Quantifying the Interaction between Copper-Zinc Superoxide Dismutase (Sod1) and its Copper Chaperone (Ccs1)},
  author={Stefanie D. Boyd and Li da Liu and Lee A. Bulla and Duane D Winkler},
  booktitle={Journal of proteomics & bioinformatics},
  year={2018}
}
Immature copper-zinc superoxide dismutase (Sod1) is activated by its copper chaperone (Ccs1). Ccs1 delivers a single copper ion and catalyzes oxidation of an intra-subunit disulfide bond within each Sod1 monomer through a mechanistically ambiguous process. Here, we use residue specific fluorescent labeling of immature Sod1 to quantitate the thermodynamics of the Sod1•Ccs1 interaction while determining a more complete view of Ccs1 function. Ccs1 preferentially binds a completely immature form of… CONTINUE READING
1
Twitter Mention

Similar Papers

Figures and Topics from this paper.

References

Publications referenced by this paper.
SHOWING 1-10 OF 33 REFERENCES

Human superoxide dismutase 1 (hSOD1) maturation through interaction with human copper chaperone for SOD1 (hCCS).

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2012
VIEW 1 EXCERPT