Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing.

@article{Hendel2014QuantifyingGO,
  title={Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing.},
  author={Ayal Hendel and Eric J Kildebeck and Eli J. Fine and J. David Clark and Niraj Punjya and Vittorio Sebastiano and Gang Bao and Matthew H Porteus},
  journal={Cell reports},
  year={2014},
  volume={7 1},
  pages={293-305}
}
Targeted genome editing with engineered nucleases has transformed the ability to introduce precise sequence modifications at almost any site within the genome. A major obstacle to probing the efficiency and consequences of genome editing is that no existing method enables the frequency of different editing events to be simultaneously measured across a cell population at any endogenous genomic locus. We have developed a method for quantifying individual genome-editing outcomes at any site of… CONTINUE READING
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