Quantifying X-ray radiation damage in protein crystals at cryogenic temperatures.

  title={Quantifying X-ray radiation damage in protein crystals at cryogenic temperatures.},
  author={Jan Kmetko and Naji S. Husseini and Matthew Naides and Yevgeniy V Kalinin and Robert E. Thorne},
  journal={Acta crystallographica. Section D, Biological crystallography},
  volume={62 Pt 9},
The dependence of radiation damage to protein crystals at cryogenic temperatures upon the X-ray absorption cross-section of the crystal has been examined. Lysozyme crystals containing varying heavy-atom concentrations were irradiated and diffraction patterns were recorded as a function of the total number of incident photons. An experimental protocol and a coefficient of sensitivity to absorbed dose, proportional to the change in relative isotropic B factor, are defined that together yield a… CONTINUE READING

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