Quantifying Trace Amounts of Aggregates in Biopharmaceuticals Using Analytical Ultracentrifugation Sedimentation Velocity: Bayesian Analyses and F Statistics

@article{Wafer2016QuantifyingTA,
  title={Quantifying Trace Amounts of Aggregates in Biopharmaceuticals Using Analytical Ultracentrifugation Sedimentation Velocity: Bayesian Analyses and F Statistics},
  author={Lucas Wafer and Marek A Kloczewiak and Yin Ling Luo},
  journal={The AAPS Journal},
  year={2016},
  volume={18},
  pages={849-860}
}
  • Lucas Wafer, Marek A Kloczewiak, Yin Ling Luo
  • Published 2016
  • Medicine, Chemistry
  • The AAPS Journal
  • Analytical ultracentrifugation–sedimentation velocity (AUC-SV) is often used to quantify high molar mass species (HMMS) present in biopharmaceuticals. Although these species are often present in trace quantities, they have received significant attention due to their potential immunogenicity. Commonly, AUC-SV data is analyzed as a diffusion-corrected, sedimentation coefficient distribution, or c(s), using SEDFIT to numerically solve Lamm-type equations. SEDFIT also utilizes maximum entropy or… CONTINUE READING

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