Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy

Abstract

We present a simple and versatile single-molecule-based method for the accurate determination of binding rates to surfaces or surface bound receptors. To quantify the reversible surface attachment of fluorescently labeled molecules, we have modified previous schemes for fluorescence correlation spectroscopy with total internal reflection illumination (TIR… (More)
DOI: 10.1021/acs.nanolett.8b00875