Quantifying Aotus monkey cytokines by real-time quantitative RT-PCR.

  title={Quantifying Aotus monkey cytokines by real-time quantitative RT-PCR.},
  author={Yago Pico de Coa{\~n}a and Carlos Alberto Barrero and Isabela Cajiao and Catalina Mosquera and Manuel Elkin Patarroyo and Manuel Alfonso Patarroyo},
  volume={27 4-5},
Aotus spp. monkeys are considered the ideal model for studying the progress of malarial infection and the immune response it elicits. We describe the use of a recently developed technique, real-time quantitative RT-PCR, to quantify several Aotus monkey cytokine mRNAs involved in Th1/Th2 responses (IL-4, IL-10, TNF-beta and IFN-gamma). Specific primers were designed for each cytokine and standard curves were constructed using serial dilutions of pDNA containing each target sequence. Results were… CONTINUE READING