Quantification of viral inactivation by photochemical treatment with amotosalen and UV A light, using a novel polymerase chain reaction inhibition method with preamplification.

@article{Allain2006QuantificationOV,
  title={Quantification of viral inactivation by photochemical treatment with amotosalen and UV A light, using a novel polymerase chain reaction inhibition method with preamplification.},
  author={J-P Allain and Jocelyn C Hsu and Manisha Pranmeth and Deborah Hanson and Adonis Stassinopoulos and Lucia Fischetti and Laurence Corash and Lily Lin},
  journal={The Journal of infectious diseases},
  year={2006},
  volume={194 12},
  pages={1737-44}
}
BACKGROUND In evaluating a photochemical treatment process for inactivating parvovirus B19, there lacked simple culture methods to measure infectivity. The recently developed enzyme-linked immunospot (ELISpot) infectivity assay uses late-stage erythropoietic progenitor cells and is labor intensive and time consuming. We evaluated a novel, efficient polymerase chain reaction (PCR) inhibition assay and examined correlations with reductions in infectivity. METHODS Contaminated plasma was treated… CONTINUE READING

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Inactivation of parvovirus B19 in platelets by helinx technology

L Sawyer, D Hanson, K. Dupuis
Transfusion • 2004
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