Quantification of total bacteria, enterobacteria and lactobacilli populations in pig digesta by real-time PCR.

@article{Castillo2006QuantificationOT,
  title={Quantification of total bacteria, enterobacteria and lactobacilli populations in pig digesta by real-time PCR.},
  author={M Z{\'u}{\~n}iga Castillo and Susana Mar{\'i}a Mart{\'i}n-Or{\'u}e and Edgar Garcia Manzanilla and Ignacio Badiola and Margarita Mart{\'i}n and Josep Gasa},
  journal={Veterinary microbiology},
  year={2006},
  volume={114 1-2},
  pages={165-70}
}
Jejunum digesta samples were taken from weaning pigs in order to evaluate real-time PCR (qPCR) as a method for quantifying pig gut bacteria. Total bacteria, lactobacilli and enterobacteria were quantified by qPCR and the results were compared with those obtained with traditional methods: 4',6-diamidino-2-phenylindole (DAPI staining) for total bacteria, selective culture for lactobacilli and enterobacteria. Real-time PCR showed higher values in terms of 16S rRNA gene copies than DAPI counts or… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 85 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 23 references

Similar Papers

Loading similar papers…