Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry.

@article{Zhang2010QuantificationOT,
  title={Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry.},
  author={Haoyue H Zhang and Dora Il'yasova and Judit Szt{\'a}ray and Sarah P. Young and Frances M Wang and David S. Millington},
  journal={Analytical biochemistry},
  year={2010},
  volume={399 2},
  pages={302-4}
}
F(2)-isoprostanes are useful biomarkers of oxidative status in humans. We developed an ultraperformance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method to quantify 2,3-dinor-8-iso prostaglandin F(2alpha), a urinary metabolite of 8-iso-prostaglandin F(2alpha.) Urine was purified by solid-phase extraction and analyzed by UPLC-MS/MS with… CONTINUE READING