Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED‐induced fluorescence detection

@article{Hhner2015QuantificationOR,
  title={Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED‐induced fluorescence detection},
  author={Jens H{\"u}hner and {\'A}lvaro Ingl{\'e}s-Prieto and Christian Neus{\"u}ss and Michael L{\"a}mmerhofer and Harald Janovjak},
  journal={ELECTROPHORESIS},
  year={2015},
  volume={36}
}
Cultured mammalian cells essential are model systems in basic biology research, production platforms of proteins for medical use, and testbeds in synthetic biology. Flavin cofactors, in particular flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), are critical for cellular redox reactions and sense light in naturally occurring photoreceptors and optogenetic tools. Here, we quantified flavin contents of commonly used mammalian cell lines. We first compared three procedures for… Expand
Bioenergetic Alterations of Metabolic Redox Coenzymes as NADH, FAD and FMN by Means of Fluorescence Lifetime Imaging Techniques
TLDR
The FLIRR approach was extended and new results were presented, which includes FLIM data of the various enzymes, such as NAD(P)H, FAD, as well as flavin mononucleotide (FMN), which used to calculate the fluorescence lifetime induced redox ratio (FLIRR), which was found to be highest for tumor and normal cells. Expand
Flavin nucleotides act as electron shuttles mediating reduction of the [2Fe‐2S] clusters in mitochondrial outer membrane protein mitoNEET
TLDR
The results suggest that flavin nucleotides may act as electron shuttles to reduce the mitoNEET [2Fe‐2S] clusters and regulate mitochondrial functions in human cells. Expand
Self-assembly of flavin mononucleotide and a cationic polythiophene in aqueous media: spectroscopic studies and sensing applications
Flavin mononucleotide (FMN) is well known as a cofactor of riboflavin and plays key roles in metabolisms. Self-assembly is one of the most important approaches for maintaining the biologicalExpand
Extracellular pH affects the fluorescence lifetimes of metabolic co-factors
TLDR
Autofluorescence measurements of the metabolic cofactors NADH and flavin adenine dinucleotide provide a label-free method to quantify cellular metabolism and changes in endogenous fluorescence lifetimes with extracellular pH are mostly due to indirect changes within the cell rather than direct pH quenching of the endogenous molecules. Expand
Fluorescence intensity and lifetime redox ratios detect metabolic perturbations in T cells.
TLDR
While both the fluorescence lifetime and intensity redox ratios resolve metabolic perturbations in T cells, the endpoints are influenced by different metabolic processes. Expand
Identification of Flavin Mononucleotide as a Cell-Active Artificial N6 -Methyladenosine RNA Demethylase.
TLDR
The first identification of a small-molecule modulator that functions as an artificial m6 A demethylase is reported, which sheds light on the development of powerful small molecules as RNA demethylases and new probes for use in RNA biology. Expand
Crystal structures of FMN‐bound and FMN‐free forms of dihydroorotate dehydrogenase from Trypanosoma brucei
TLDR
Comparisons of B factors of the protein main chain revealed that binding of FMN decreased flexibility of most of the residues at theFMN‐binding site, but increased flexibility of a lid‐like loop structure over the active center, which was ascribed to a conformational change in an FMN‐contacting residue, Asn195, which induced a rearrangement of a hydrogen‐bond network of the residue comprising the lid. Expand
Ultrasensitive determination for flavin coenzyme by using a ZnO nanorod photoelectrode in a four-electrode system
AbstractA photoelectrochemical sensor for FAD and FMN was fabricated by incorporating the reduced flavin cofactor as an electron donor into a photoelectrochemical reaction in a ZnO nanorodExpand
The mitochondrial outer membrane protein mitoNEET is a redox enzyme catalyzing electron transfer from FMNH2 to oxygen or ubiquinone
TLDR
The results suggest that mitoNEET is a redox enzyme that may promote oxidation of NADH to facilitate enhanced glycolysis in the cytosol and that pioglitazone may regulate energy metabolism in mitochondria by inhibiting the electron transfer activity of mito NEET. Expand
The superfamily keeps growing: Identification in trypanosomatids of RibJ, the first riboflavin transporter family in protists
TLDR
The essentiality of riboflavin for trypanosomatids, and the structural/biochemical differences that RFVT/SLC52 and RibJ present, make the rib oflavin transporter -and its downstream metabolism- a potential trypanocidal drug target. Expand
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