Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy.

@article{Zheng1999QuantificationOR,
  title={Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy.},
  author={Yuan Zhi Zheng and Alex Hyatt and Lin-fa Wang and Bryan T. Eaton and Paul F. Greenfield and Steven Reid},
  journal={Journal of virological methods},
  year={1999},
  volume={80 1},
  pages={1-9}
}
Immunosorbent electron microscopy was used to quantify recombinant baculovirus-generated bluetongue virus (BTV) core-like particles (CLP) in either purified preparations or lysates of recombinant baculovirus-infected cells. The capture antibody was an anti-BTV VP7 monoclonal antibody. The CLP concentration in purified preparations was determined to be 6.6 x… CONTINUE READING