Quantification of proteolytically active pregnancy-associated plasma protein-A with an assay based on quenched fluorescence.


BACKGROUND Maternal serum concentrations of pregnancy-associated plasma protein-A (PAPP-A, pappalysin-1, EC are used to predict the occurrence of Down syndrome. In pregnancy, PAPP-A primarily circulates as a covalent 2:2 complex with the proform of eosinophil major basic protein (proMBP), which inhibits the proteolytic activity of PAPP-A. At term… (More)


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