Quantification of proteins using (13)C7-labeled and unlabeled iodoacetanilide by nano liquid chromatography/nanoelectrospray ionization and by selected reaction monitoring mass spectrometry.

@article{Kurono2015QuantificationOP,
  title={Quantification of proteins using (13)C7-labeled and unlabeled iodoacetanilide by nano liquid chromatography/nanoelectrospray ionization and by selected reaction monitoring mass spectrometry.},
  author={S. Kurono and Yuka Kaneko and Shuji Matsuura and S. Niwayama},
  journal={Bioorganic & medicinal chemistry letters},
  year={2015},
  volume={25 5},
  pages={
          1110-6
        }
}
The combination of cysteine-specific modifiers, iodoacetanilide (IAA) and (13)C7-labeled iodoacetanilide ((13)C7-IAA), has been applied to absolute quantification of proteins. The selected reaction monitoring (SRM) with the use of nano liquid chromatography/nanoelectrospray ionization ion trap mass spectrometry (nano LC/nano-ESI-IT-MS) analysis was applied to precise quantification of three commercial proteins. Good correlation was observed between the theoretical ratios and observed ratios for… Expand

References

SHOWING 1-10 OF 24 REFERENCES
An approach to quantitative proteome analysis by labeling tryptophan residues.
Peptide peak intensities enhanced by cysteine modifiers and MALDI TOF MS.
Quantitative analysis of mTRAQ-labeled proteome using full MS scans.
...
1
2
3
...