Quantification of protein in dilute and complex samples: modification of the bicinchoninic acid assay.

Abstract

The colorimetric assay using bicinchoninic acid (BCA) as test reagent is useful for quantitative protein determinations due to its high sensitivity, ease, and tolerance to various contaminations present in biological samples or added during purification. For removal of interfering substances, protein precipitations have been described. Yet, obstructions became apparent with diluted and complex samples. Therefore we tested different solvents for removal of such interfering contaminants from the protein precipitate, and 1 M HCl was identified as the useful washing agent. The protocol described allows simple and accurate microdetermination in microtiter plates of proteins from complex samples.

02040201520162017
Citations per Year

Citation Velocity: 6

Averaging 6 citations per year over the last 3 years.

Learn more about how we calculate this metric in our FAQ.

Cite this paper

@article{Schoel1995QuantificationOP, title={Quantification of protein in dilute and complex samples: modification of the bicinchoninic acid assay.}, author={Bernd Schoel and Morgan Welzel and Stefan H C Kaufmann}, journal={Journal of biochemical and biophysical methods}, year={1995}, volume={30 2-3}, pages={199-206} }