Quantification of protein backbone hydrogen-deuterium exchange rates by solid state NMR spectroscopy.

  title={Quantification of protein backbone hydrogen-deuterium exchange rates by solid state NMR spectroscopy.},
  author={Juan-Miguel Lopez del Amo and Uwe Fink and Bernd Reif},
  journal={Journal of biomolecular NMR},
  volume={48 4},
We present the quantification of backbone amide hydrogen-deuterium exchange rates (HDX) for immobilized proteins. The experiments make use of the deuterium isotope effect on the amide nitrogen chemical shift, as well as on proton dilution by deuteration. We find that backbone amides in the microcrystalline α-spectrin SH3 domain exchange rather slowly with the solvent (with exchange rates negligible within the individual (15)N-T (1) timescales). We observed chemical exchange for 6 residues with… CONTINUE READING
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