Quantification of propidium iodide delivery using millisecond electric pulses: experiments.

@article{Sadik2013QuantificationOP,
  title={Quantification of propidium iodide delivery using millisecond electric pulses: experiments.},
  author={Mohamed M. Sadik and Jianbo Li and Jerry W. Shan and David I Shreiber and Hao Lin},
  journal={Biochimica et biophysica acta},
  year={2013},
  volume={1828 4},
  pages={
          1322-8
        }
}
The transport mechanisms in electroporation-mediated molecular delivery are experimentally investigated and quantified. In particular, the uptake of propidium iodide (PI) into single 3T3 fibroblasts is investigated with time- and space-resolved fluorescence microscopy, and as a function of extracellular buffer conductivity. During the pulse, both the peak and the total integrated fluorescence intensity exhibit an inverse correlation with extracellular conductivity. This behavior can be… CONTINUE READING

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