Quantification of plasma cell-free DNA in patients with gastric cancer.

@article{Sai2007QuantificationOP,
  title={Quantification of plasma cell-free DNA in patients with gastric cancer.},
  author={Soujin Sai and Daisuke Ichikawa and Haruhisa Tomita and Daito Ikoma and Nobuyuki Tani and Hisashi Ikoma and Shojiro Kikuchi and Hitoshi Fujiwara and Yuji Ueda and Eigo Otsuji},
  journal={Anticancer research},
  year={2007},
  volume={27 4C},
  pages={2747-51}
}
BACKGROUND The circulating DNA concentration and integrity was examined by a quantitative polymerase chain reaction (qPCR) in the plasma from patients with gastric cancer and their diagnostic value for the detection of gastric cancer assessed. PATIENTS AND METHODS Plasma samples were collected preoperatively from 53 patients with gastric cancer and 21 healthy controls. qPCR was performed using two different primer sets for the beta-actin gene, amplifying short and long segments. DNA integrity… CONTINUE READING