Quantification of pancreatic islet distribution in situ in mice.

@article{Kilimnik2009QuantificationOP,
  title={Quantification of pancreatic islet distribution in situ in mice.},
  author={German Kilimnik and Abraham Kim and Junghyo Jo and Kevin M. Miller and Manami Hara},
  journal={American journal of physiology. Endocrinology and metabolism},
  year={2009},
  volume={297 6},
  pages={E1331-8}
}
Tracing changes of specific cell populations in health and disease is an important goal of biomedical research. Precisely monitoring pancreatic beta-cell proliferation and islet growth is a challenging area of research. We have developed a method to capture the distribution of beta-cells in the intact pancreas of transgenic mice with fluorescence-tagged beta-cells with a macro written for ImageJ (rsb.info.nih.gov/ij/). Total beta-cell area and islet number and size distribution are quantified… CONTINUE READING

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