Quantification of obeche wood allergen: Development of a sensitive sandwich-ELISA for occupational exposure assessment.

@article{Kespohl2016QuantificationOO,
  title={Quantification of obeche wood allergen: Development of a sensitive sandwich-ELISA for occupational exposure assessment.},
  author={Sabine Kespohl and Paloma Campo and Eva Zahradnik and Silke Maryska and A Aranda-Guerrero and Juan Eduardo Rodriguez and Thomas Br{\"u}ning and Monika Raulf},
  journal={Journal of toxicology and environmental health. Part A},
  year={2016},
  volume={79 22-23},
  pages={1070-1077}
}
Obeche wood is a prominent cause of allergic occupational asthma. To reduce the risk of immunoglobulin E (IgE)-mediated sensitization it is important to assess airborne obeche wood allergen concentrations at exposed workplaces. Therefore, a highly sensitive obeche wood allergen immunoassay was developed and applicability was proven on airborne passive dust samples in Spanish wood workshops. Obeche wood sandwich enzyme-linked immunosorbent assay (ELISA) polyclonal antibodies (pAbs) were… CONTINUE READING