Quantification of nucleotide-activated sialic acids by a combination of reduction and fluorescent labeling.

  title={Quantification of nucleotide-activated sialic acids by a combination of reduction and fluorescent labeling.},
  author={S. Galuska and H. Geyer and B. Weinhold and M. Kontou and Ren{\'e} C R{\"o}hrich and U. Bernard and R. Gerardy-Schahn and W. Reutter and A. M{\"u}nster-K{\"u}hnel and R. Geyer},
  journal={Analytical chemistry},
  volume={82 11},
  • S. Galuska, H. Geyer, +7 authors R. Geyer
  • Published 2010
  • Chemistry, Medicine
  • Analytical chemistry
  • Sialic acids usually represent the terminal monosaccharide of glycoconjugates and are directly involved in many biological processes. The cellular concentration of their nucleotide-activated form is one pacemaker for the highly variable sialylation of glycoconjugates. Hence, the determination of CMP-sialic acid levels is an important factor to understand the complex glycosylation machinery of cells and to standardize the production of glycotherapeutics. We have established a highly sensitive… CONTINUE READING
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