A novel approach to decrease sialic acid expression in cells by a C-3-modified N-acetylmannosamine.
- Paul Robin Wratil, Stephan Rigol, +5 authors Long Duc Nguyen
- The Journal of biological chemistry
- 2014
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Quantification of nucleotide-activated sialic acids by a combination of reduction and fluorescent labeling.
@article{Galuska2010QuantificationON,
title={Quantification of nucleotide-activated sialic acids by a combination of reduction and fluorescent labeling.},
author={Sebastian P Galuska and Hildegard Geyer and Birgit Weinhold and Maria Kontou and Ren{\'e} C R{\"o}hrich and Ulrike Bernard and Rita Gerardy-Schahn and Werner Reutter and Anja K. M{\"u}nster-K{\"u}hnel and Robert P. Geyer},
journal={Analytical chemistry},
year={2010},
volume={82 11},
pages={
4591-8
}
}- Published in Analytical chemistry 2010
DOI:10.1021/ac100627e
Sialic acids usually represent the terminal monosaccharide of glycoconjugates and are directly involved in many biological processes. The cellular concentration of their nucleotide-activated form is one pacemaker for the highly variable sialylation of glycoconjugates. Hence, the determination of CMP-sialic acid levels is an important factor to understand the complex glycosylation machinery of cells and to standardize the production of glycotherapeutics. We have established a highly sensitive… CONTINUE READING