Quantification of nitrotyrosine in nitrated proteins

@inproceedings{Yang2010QuantificationON,
  title={Quantification of nitrotyrosine in nitrated proteins},
  author={Hong Yang and Yingyi Zhang and Ulrich P{\"o}schl},
  booktitle={Analytical and bioanalytical chemistry},
  year={2010}
}
For kinetic studies of protein nitration reactions, we have developed a method for the quantification of nitrotyrosine residues in protein molecules by liquid chromatography coupled to a diode array detector of ultraviolet-visible absorption. Nitrated bovine serum albumin (BSA) and nitrated ovalbumin (OVA) were synthesized and used as standards for the determination of the protein nitration degree (ND), which is defined as the average number of nitrotyrosine residues divided by the total number… CONTINUE READING

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