Quantification of nano-scale intermembrane contact areas by using fluorescence resonance energy transfer.

@article{Bendix2009QuantificationON,
  title={Quantification of nano-scale intermembrane contact areas by using fluorescence resonance energy transfer.},
  author={Poul Martin Bendix and Mette Storgaard Pedersen and Dimitrios Stamou},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2009},
  volume={106 30},
  pages={12341-6}
}
Nanometer-scale intermembrane contact areas (CAs) formed between single small unilamellar lipid vesicles (SUVs) and planar supported lipid bilayers are quantified by measuring fluorescence resonance energy transfer (FRET) between a homogenous layer of donor fluorophores labeling the supported bilayer and acceptor fluorophores labeling the SUVs. The smallest CAs detected in our setup between biotinylated SUVs and dense monolayers of streptavidin were approximately 20 nm in radius. Deformation of… CONTINUE READING

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