Quantification of metabolically active biomass using Methylene Blue dye Reduction Test (MBRT): measurement of CFU in about 200 s.

@article{Bapat2006QuantificationOM,
  title={Quantification of metabolically active biomass using Methylene Blue dye Reduction Test (MBRT): measurement of CFU in about 200 s.},
  author={Prashant Madhusudan Bapat and Subir K. Nandy and Pramod P. Wangikar and K. V. Venkatesh},
  journal={Journal of microbiological methods},
  year={2006},
  volume={65 1},
  pages={107-16}
}
Quantification of viable cells is a critical step in almost all biological experiments. Despite its importance, the methods developed so far to differentiate between viable and non-viable cells suffer from major limitations such as being time intensive, inaccurate and expensive. Here, we present a method to quantify viable cells based on reduction of methylene blue dye in cell cultures. Although the methylene blue reduction method is well known to check the bacterial load in milk, its… CONTINUE READING

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Quantification of metabolically active biomass using Methylene Blue dye Reduction Test ( MBRT ) : measurement of CFU in about 200 s. Quantification of viable cells is a critical step in almost all biological experiments .
Here , we present a method to quantify viable cells based on reduction of methylene blue dye in cell cultures .
The Methylene Blue dye Reduction Test ( MBRT ) correlates very well with Colony Forming Units ( CFU ) up to a 800 live cells as established by plating .
The Methylene Blue dye Reduction Test ( MBRT ) correlates very well with Colony Forming Units ( CFU ) up to a 800 live cells as established by plating .
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