Quantification of mRNA using real-time RT-PCR

@article{Nolan2006QuantificationOM,
  title={Quantification of mRNA using real-time RT-PCR},
  author={Tania Nolan and Rebecca E Hands and Stephen A Bustin},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={1559-1582}
}
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described as a “gold” standard, it is far from being a standard assay. The significant problems caused by variability of RNA templates, assay designs and protocols, as well as inappropriate data… 

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