Quantification of mRNA of tyrosine hydroxylase and aromatic L-amino acid decarboxylase in the substantia nigra in Parkinson's disease and schizophrenia.

@article{Ichinose1994QuantificationOM,
  title={Quantification of mRNA of tyrosine hydroxylase and aromatic L-amino acid decarboxylase in the substantia nigra in Parkinson's disease and schizophrenia.},
  author={Hiroshi Ichinose and Tamae Ohye and Kiyotaka Fujita and F. Pantucek and Kezia Lange and Peter Franz Riederer and Toshiharu Toshi Nagatsu},
  journal={Journal of neural transmission. Parkinson's disease and dementia section},
  year={1994},
  volume={8 1-2},
  pages={149-58}
}
Using the reverse transcription-polymerase chain reaction (RT-PCR), we developed a sensitive and quantitative method to detect all four types of human tyrosine hydroxylase (TH) mRNAs in the human brain (substantia nigra). All four types of TH mRNAs were found in the substantia nigra in the control brains examined, and the ratio of type-1, type-2, type-3, and type-4 mRNAs to the total amount of TH was 45, 52, 1.4, and 2.1%, respectively. The average amount of total TH mRNA in the normal brain… CONTINUE READING