Quantification of mRNA in single oocytes and embryos by real-time rapid cycle fluorescence monitored RT-PCR.

Abstract

Deciphering the complex series of regulatory events that occur during early development depends partly on the ability to accurately quantify stage-specific mRNA species. However, the paucity of biological material coupled with the lack of sensitivity and/or reproducibility of the currently available quantitative methods had been severe limitations on single… (More)

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@article{Steuerwald2000QuantificationOM, title={Quantification of mRNA in single oocytes and embryos by real-time rapid cycle fluorescence monitored RT-PCR.}, author={Nury M. Steuerwald and J. Cohen and R. J. Herrera and C. A. Brenner}, journal={Molecular human reproduction}, year={2000}, volume={6 5}, pages={448-53} }