Quantification of lipid peroxidation in tissue extracts based on Fe(III)xylenol orange complex formation.

@article{HermesLima1995QuantificationOL,
  title={Quantification of lipid peroxidation in tissue extracts based on Fe(III)xylenol orange complex formation.},
  author={Marcelo Hermes-Lima and William. G. Willmore and Kenneth B. Storey},
  journal={Free radical biology & medicine},
  year={1995},
  volume={19 3},
  pages={271-80}
}
Commonly used spectrophotometric methods for determining the extent of lipid peroxidation in animal tissue extracts, such as measurements of diene conjugation and thiobarbituric acid reactive substances (TBARS), have been criticized for their lack of specificity. This study shows that lipid hydroperoxides can be effectively quantified in animal tissue extracts using an assay based on the formation of a Fe(III)xylenol orange complex. Addition of H2O2, cumene hydroperoxides, or methanolic tissue… CONTINUE READING
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