Quantification of isotope encoded proteins in 2-D gels using surface enhanced resonance Raman.

Abstract

A strategy for quantification of multiple protein isoforms from a complex sample background is demonstrated, combining isotopomeric rhodamine 6G (R6G) labels and surface-enhanced Raman in polyacrylamide matrix. The procedure involves isotope-encoding by lysine-labeling with (R6G) active ester reagents, isoform separation by 2-DGE, fluorescence… (More)
DOI: 10.1021/bc800325k

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Cite this paper

@article{Knudsen2008QuantificationOI, title={Quantification of isotope encoded proteins in 2-D gels using surface enhanced resonance Raman.}, author={Giselle M. Knudsen and Brandon M. Davis and Shirshendu K. Deb and Yvette L. Loethen and Ravindra K Gudihal and Pradeep Rasika Perera and Dor Ben-Amotz and V. Jo Davisson}, journal={Bioconjugate chemistry}, year={2008}, volume={19 11}, pages={2212-20} }