Quantification of intact 146S foot-and-mouth disease antigen for vaccine production by a double antibody sandwich ELISA using monoclonal antibodies.

@article{Maanen1990QuantificationOI,
  title={Quantification of intact 146S foot-and-mouth disease antigen for vaccine production by a double antibody sandwich ELISA using monoclonal antibodies.},
  author={C. van Maanen and Catharinus Terpstra},
  journal={Biologicals : journal of the International Association of Biological Standardization},
  year={1990},
  volume={18 4},
  pages={
          315-9
        }
}
  • C. van Maanen, Catharinus Terpstra
  • Published in
    Biologicals : journal of the…
    1990
  • Biology, Medicine
  • A double antibody sandwich (DAS) enzyme-linked immunosorbent assay (ELISA) was developed to quantify 146S antigen of foot-and-mouth disease virus (FMDV) strain A10 Holland grown in suspension cultures of surviving bovine tongue epithelium. When virus harvests were incubated with trypsin--which affects VP1, the most immunogenic structural protein of FMDV--the concentration of 146S antigen as determined by ELISA was reduced by greater than 90%. Therefore, the test detected essentially only those… CONTINUE READING

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