Quantification of inhibin/activin alpha and betaA subunit messenger ribonucleic acid by competitive reverse transcription-polymerase chain reaction in chicken granulosa cells during follicular development.

Abstract

The very sensitive quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the expression of inhibin/activin subunits in the granulosa cells of developing ovarian follicles of the hen. Two competitors specific to inhibin alpha and betaA subunits were constructed. In one study, the expression of inhibin alpha… (More)

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